《植物生理学报》 2014, 50(10): 1541-1545

北美冬青‘奥斯特’的组织培养和快速繁殖

张俊林¹, 余有祥², 沈柏春¹, 丁旭升¹, 钱飞¹, 孙英坤¹, 吴光洪^1,*

¹杭州市园林绿化股份有限公司, 杭州310000; ²杭州润土园艺科技有限公司, 杭州310000

通信作者：吴光洪；E-mail: 51576419@qq.com；Tel: 0571-86020113

摘　要：

以北美冬青‘奥斯特’ (‘Oosterwijk’)成年雌性优良单株半木质化茎段为材料, 建立了组织培养快速繁殖体系。结果表明, WPM+6-BA 0.50 mg•L^-1+NAA 0.05 mg•L^-1可作为启动培养基, 最佳的继代增殖培养基为WPM+6-BA 1.00 mg•L^-1+NAA 0.10 mg•L^-1+蔗糖20 g•L^-1, 25 d的增殖系数为6.1; 生根培养基为1/2WPM+IBA 0.20 mg•L^-1+NAA 0.40 mg•L^-1, 生根率达95.2%, 每株平均生根5.7条。经生根培养30 d和日光温室炼苗10 d后, 试管苗移入泥炭和珍珠岩(1:1, V/V)混合基质中, 移栽后的成活率达98%。该体系的建立为北美冬青规模化生产提供了技术平台。

关键词：北美冬青; 组织培养; 快速繁殖

收稿：2014-07-21   修定：2014-08-20

资助：中央财政林业科技推广示范项目([2012]TS008号)。

Tissue Culture and Rapid Propagation of Ilex verticillata ‘Oosterwijk’

ZHANG Jun-Lin¹, YU You-Xiang², SHEN Bo-Chun¹, Ding Xu-Sheng¹, QIAN Fei¹, SUN Ying-Kun¹, WU Guang-Hong^1,*

¹Hangzhou Landscaping Incorporated, Hangzhou 310000, China, ²Hangzhou RUN-TU Gardening Science and Technology Co., Ltd., Hangzhou 310000, China

Corresponding author: WU Guang-Hong; E-mail: 51576419@qq.com; Tel: 0571-86020113

Abstract:

The tissue culture technology was studied in this research by using semi-lignified shoots from female individual ‘Oosterwijk’ (Ilex verticillata). The results showed that bud formation on the nodal explants was induced on the WPM medium supplementing with 6-BA 0.50 mg•L^-1 and NAA 0.05 mg•L^-1; the best medium for proliferation was WPM supplemented with 6-BA 1.00 mg•L^-1, NAA 0.10 mg•L^-1 and sucrose 20 g•L^-1, the proliferation coefficient was 6.1 after subculturing for 25 d; the optimal rooting medium was 1/2WPM supplemented with IBA 0.20 mg•L^-1 and NAA 0.40 mg•L^-1, with rooting rate up to 95.2% and 5.7 roots per shoot. After rooting culture for 30 days and acclimatization in greenhouse for 10 days, the shoots were transferred to the substrate of perlite and peaty soil (1:1, V/V) and the survival rate was 98%. These results can provide the technical basis for scale production of plantlets of I. verticillata ‘Oosterwijk’.

Key words: Ilex verticillata; tissue culture; rapid propagation